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Design: Baseline microbiota of hematopoietic stem cell transplant donors and recipients
Submitted by: University of California San Diego Microbiome Initiative (University of California San Diego Microbiome Init)
Study: Baseline Fecal Samples of Hemapoetic Stem Cell Transplant Donors and Patients
PRJEB16057 • ERP017899 • All experiments • All runs
show Abstracthide Abstract
We evaluated whether the baseline intestinal microbiota (16S-rRNA) of hematopoietic stem cell transplant patients and donors are implicated in transplant outcomes--namely, acute gastrointestinal graft-versus-host disease (GVHD).
Library:
Name: 10564.TPC4DXSR
Instrument: Illumina MiSeq
Strategy: OTHER
Source: METAGENOMIC
Selection: PCR
Layout: SINGLE
Construction protocol: Extracted DNA was normalized ~10^6 template/microliter, then primers 534F (GTGCCAGCMGCCGCGGTAA) and 806R (GGACTACHVGGGTWTCTAAT) were used for targeted amplification of the V4 region of 16S-rRNA for Illumina paired-end sequencing.
Experiment attributes: (show all 11 attributes...) (hide...)
center_name: Daniel Frank Laboratory, University of Colorado, A (show full text...)(hide...)
Daniel Frank Laboratory, University of Colorado, Anschutz
center_project_name: Stem cell
pcr_primers: FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
primer: GTGCCAGCMGCCGCGGTAA
run_center: Daniel Frank Laboratory, University of Colorado, A (show full text...)(hide...)
Daniel Frank Laboratory, University of Colorado, Anschutz
run_date: 2015
run_prefix: TPC4DXSR-R1
sample_center: UC-Anschutz
sequencing_meth: sequencing by synthesis
target_gene: 16S rRNA
target_subfragment: V4
Run# of Spots# of BasesSizePublished
ERR1688300unavailable2016-10-18

ID:
3300726

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